Abstract. The dilution of antibodies for stock and working solutions are critical for both proper storage and successful experiments. Here is a comprehensive discussion of antibody dilution and how dilutions are presented in the literature. Additionally there is a brief discussion on the related topic of antibody titer. Another way to look at this is to calculate the dilution factor, that is, the ratio between the initial and final concentrations. Diluting a 5M solution to a 5mM solution is a 1000-fold dilution: c 5mM 1M 1. 2 = × =. c1 5M 1000mM 1000. Therefore, you need 1 part stock solution to make 1000 parts of your final solution. c. You can treat a ratio as a fraction or a division problem: 1:4 = 1 / 4 = 1 ÷ 4. Solve this problem with long division (or a calculator) and you'll get the answer as a decimal: 0.25. To make this a percent, just move the decimal point two spaces to the right: 0.25 = 25%. Thanks! The original volume of your solution was 2 mL, and the final volume was 12 mL, so the dilution factor is simply 2 12 2 12. The original concentration times the dilution factor gives the final concentration: 10 mg L ⋅ 2 12 =1.7 mg L 10 m g L ⋅ 2 12 = 1.7 m g L. Note that only 2 significant figures were given in the problem and your answer To convert dilution ratios to ounces per gallon divide 128 (the number of ounces per gallon) by the dilution ratio. For example, for a dilution rate of 1:32, the ounces per gallon would be determined by the calculation: 128 ÷ 32 = 4-ounces per gallon of water. If given the ounces per gallon, calculate the dilution rate by dividing the Calculate the final dilution ratio in a serial dilution. The total dilution ratio can be determined by multiplying the dilution factor of each step leading up to the final step. This can be mathematically illustrated with the equation D t = D 1 x D 2 x D 3 x … x D n where D t is the total dilution factor and D n is the dilution ratio. diluted solution, dilution factor as a ratio of volumes, 1:250 So, V 1 = 1 and V 2 = 250; c final = concentration of Fe 2+ in diluted solution = 1.62 x 10-5 mol L-1; PAUSE! Plan. Step 1. Calculate the dilution factor DF = dilution factor = V 2 ÷ V 1 = Step 2. Calculate the concentration of Fe 2+ (aq) in stock solution. c initial = DF × c Adding solvent results in a solution of lower concentration. You can calculate the concentration of a solution following a dilution by applying this equation: M i V i = M f V f. where M is molarity, V is volume, and the subscripts i and f refer to the initial and final values. To calculate how much of a weaker spirit or water you need to add to dilute your alcohol: Multiply the concentration of the weaker spirit in % (0% for water) by the volume of the stronger spirit. Divide the result by the concentration of the stronger spirit (also in %). Subtract this result from the volume of the stronger spirit. soaON.

how to calculate dilution ratio